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RxnReady™ Primer Pools
Standard DNA »
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#
Scale
Sequence 1
(5' → 3')
Bases
# Bases:
Convert to reverse complement
GC:
%
Tm:
ºC
DeltaG:
kcal/mol
Sequence 2
(5' → 3')
Bases
# Bases:
Convert to reverse complement
GC:
%
Tm:
ºC
DeltaG:
kcal/mole
Notes
Purification
Standard Desalting
Formulation
Quantity only
Quantity and volume
Quantity (nmol)
Quantity
(nmol)
Concentration
(µM)
Volume
(µL)
Buffer
IDTE (1X TE Solution) pH 8.0
IDTE (1X TE Solution) pH 7.5
RNase-Free Water (additional shipping charge required)
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Base Notations
Standard Bases, (A, C, G, T)
are not case sensitive
.
Mixed Bases, expressed in
UPPERCASE.
RNA, expressed as 'r_', for example rA, rU.
2' O-methyl RNA bases are entered as 'm_'.
Phosphorothioated DNA bases are entered as '_*'
Phosphorothioated RNA bases are entered as 'r_*'.
Phosphorothioated 2'-O-methyl RNA bases are entered as 'm_*'.
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# Entries:
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Scale
Code
Purification
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Purification
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Melting Temperature Settings
Parameter sets
SpecSheet (Default)
qPCR
Target type
DNA
RNA
Oligo Conc
µM
Na
+
Conc
mM
Mg
++
Conc
mM
dNTPs Conc
mM