CRISPR genome editing
Primer design and other tools you should know about
IDT offers a free suite of software applications—the SciTools™ Web Tools—which are available on IDT’s website. These tools give you the opportunity to determine the properties of any oligo sequence and facilitate the design and conditions of your experimental set-up.
- Primer design and other tools you should know about
- Single base editing
- Customizing CAR-T cells using the CRISPR-Cas9 system
- CRISPR knock-in: Tips and tricks
- CRISPRa and CRISPRi: Similarities and differences you should know about
- Megamer single-stranded donor templates (ssDNA or ssODNs) for successful homology-directed repair (HDR) in genome editing applications
- The CRISPR Basics Handbook: An IDT publication to help you learn CRISPR
- Genome editing tip: A CRISPR RNA annealing step can increase editing efficiency
- CRISPR GUARD: A short gRNA-based system that facilitates precise genome edits
- Alt-R™ HDR Donor Blocks can enable long DNA knock-ins while decreasing undesired edits
- Improving efficiency of homology-directed repair (HDR)
- CRISPECTOR, a statistical CRISPR genome editing analysis software for research
- A.s. Cas12a Ultra, a highly effective CRISPR genome editing enzyme
- A fusion gene identification strategy using CRISPR-Cas9 and long read nanopore sequencing
- Genome Oligopaint: Painting a clearer picture with oPools™ Oligo Pools
- Analyze your CRISPR genome edits with IDT's cloud-based software
- CRISPR-based depletion of unwanted sequences improves targeted next generation sequencing
- Applications and benefits of CRISPR functional genomics screens using arrayed guide RNA libraries
- SHERLOCK, PAC-MAN, and more: How CRISPR-Cas13 enzymes may be useful in the COVID-19 pandemic and beyond
- Stability of CRISPR reagents under different freezing, storage, and thawing conditions
- Consider ribonucleoprotein (RNP) instead of CRISPR plasmids!
- CRISPR fluorescent in situ hybridization in live cells: a new approach to monitoring chromosomal activities
- Prime editing with pegRNA: a novel and precise CRISPR genome editing system
- What is CRISPR screening?
- A mutant A.s. Cas12a (Cpf1) for CRISPR genome editing shows broad temperature tolerance and a high ratio of on:off target activity, with site-to-site reliability rivaling S.p. Cas9
- Similar editing efficiency with two-part and single guide RNA
- Using CRISPR-Cas9 technology for targeted nanopore sequencing
- High-fidelity Cas9 provides highly efficient genome editing with reduced off-target effects, even in primary cells
- When and how to use nickases for efficient genome editing
- Genome editing: Isolating clones for genotypic and phenotypic characterization
