SameDay™ DNA Oligos
Custom DNA oligos-manufactured and shipped the same day you order
SameDay Oligos are standard, desalted DNA oligos, delivered in tubes using our fastest shipping method. Place your order before the specified deadline to receive your oligos the following business day.
Ordering
- Receive your custom oligos the next business day after placing your order
- Tested by ESI-MS prior to shipment*-expedited without compromising quality
- No minimum or maximum order requirements
- Choose formulation for shipment-dry or hydrated in IDTE, pH 8
* With the exception of mixed base oligos, which will result in multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.
SameDay Oligos
Shipped dry or resuspended to 100 µM in IDTE buffer, pH 8.0. Delivered in tubes. To meet the ordering deadline, orders for SameDay Oligos must be placed online.
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Product Details
SameDay Oligos are standard desalted DNA oligos, delivered in tubes using our fastest shipping service available. If you place your order before the day's deadline (see below), you will receive your oligos on the following business day.
Every DNA Oligo you receive will be deprotected and desalted to remove small molecule impurities. In addition, your oligos will be quality controlled via proprietary ESI-mass spectrometry methods* and quantitated twice by UV spectrophotometry for precise yield measurements.
* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.
SameDay DNA Oligos must be ordered online and are no longer available by
email request.
Specifications
- 8 nmol minimum guarantee (sufficient for >250 PCR reactions)
- 15−45 DNA bases; no modifications or modified bases
- Each oligo quality controlled by ESI mass spectrometry*
- Available in tubes only, dry or hydrated in IDTE buffer, pH 8.0
- Deprotected and desalted
- Additional shipping and handling fee for expedited delivery
* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.
Order deadlines
United States
Order online by 3:00 pm ET
Europe
Order online by 1:00 pm CET
Singapore
Order online by 10:00 am SGT
Product Data
IDT's long-standing reputation as a leader and pioneer in custom oligo manufacturing is largely due to our vertically integrated proprietary synthesis platforms. Nearly every component of our manufacturing process is designed and developed in-house, including specialized synthesizers that can accommodate the most demanding oligo requests, and high-throughput automation systems that help ensure your oligos reach you when you need them. We do not rely on third-party manufacturers for any of the machines or chemical reagents used for synthesis, allowing us to monitor and refine instruments and reagents with ease. Our ability to control these variables means we can consistently produce quality oligos.
An important measure of quality for any oligo manufacturer is coupling efficiency, because this directly affects the amount of full-length product you receive. Our manufacturing processes result in high coupling efficiencies (Figure 1) and are standardized across our global manufacturing sites (Figure 2), meaning no matter where in the world you are conducting your research, an IDT oligo is an IDT oligo. Our commitment to quality and consistency results in a reliable product that helps drive your research forward.
Figure 1. IDT proprietary platforms have a better coupling efficiency than other suppliers, which provides more full-length oligonucleotides in your order. Small increases in coupling efficiency (≤1%) result in measurable increases in full-length product yield. The curves represent the theoretical yield for different lengths of oligos based on a coupling efficiency of 99.4% (IDT oligos, n = 126) and 99.1% (other suppliers, n = 134 from three different suppliers) using the formula, percent full length product = (eff )(n-1)*100 where eff = coupling efficiency (for example, 99.4% = 0.994) and (n–1) is the number of coupling reactions needed to make an oligo of length n.
Figure 2. IDT tracks coupling efficiency at our manufacturing sites to ensure consistent quality. Many variables can lead to reduced oligo quality, including ambient temperature, humidity, and input reagents. Monitoring of coupling efficiency at IDT manufacturing sites ensures that we provide our customers with high quality products. A quarterly average is shown (n = 16 per manufacturing site).
Resources
Frequently asked questions
What are the restrictions and pricing for SameDay™ Oligo Service?
SameDay Oligo Service is restricted to 25 nmol scale oligonucleotides of 15–45 bases with no modifications or additional purification.
In the USA, the oligos must be ordered by 3:00 pm Eastern Time in order to be delivered the following business day by 10:30 am. For the most up-to-date pricing, contact us.
How do I order SameDay™ Oligos?
SameDay Oligos must be ordered on our website before the SameDay deadline of 3:00 pm (US Eastern Time).
Log in to your online web account, click on SameDay Oligo Service under the Expedited Services option, and then click "Order." For information regarding SameDay service and deadlines, please visit our website and click on "SameDay Oligo Service."