RxnReady™ Primer Pools
Premixed primer pairs supplied ready to use.
All PCR and qPCR primers are produced high coupling efficiencies, resulting in high-quality DNA products. Our specialized platforms allow us to deliver you pure primers for your research needs.
Ordering
RxnReady Primer Pools
2 oligos premixed in a single tube. Shipped dry, or resuspended to your specifications.
Loading...
Loading...
Product Details
Every DNA Oligo you receive will be deprotected and desalted to remove small molecule impurities. In addition, your oligos will be quality controlled via electrospray ionization mass spectrometry (ESI-MS), and quantitated by UV spectrophotometry.
For oligos 25–50 bases, we offer the following yield guarantees*:
| Product | Available length | Guaranteed yield |
|---|---|---|
| 25 nmole DNA Oligo | 15–60 bases | 10 nmol |
| 100 nmole DNA Oligo | 10–90 bases | 30 nmol |
| 250 nmole DNA Oligo | 5–100 bases | 50 nmol |
Product Data
An important measure of quality for any oligo manufacturer is coupling efficiency, because this directly affects the amount of full-length product you receive. Our manufacturing processes result in high coupling efficiencies (Figure 1) and are standardized across our global manufacturing sites (Figure 2) to provide you with consistent quality. Our commitment to quality and consistency results in a reliable product that helps provide confidence in your research.
Figure 1. IDT proprietary platforms have a better coupling efficiency than other suppliers, which provides more full-length oligonucleotides in your order. Small increases in coupling efficiency (≤1%) result in measurable increases in full-length product yield. The curves represent the theoretical yield for different lengths of oligos based on a coupling efficiency of 99.4% (IDT oligos, n = 126) and 99.1% (other suppliers, n = 134 from three different suppliers) using the formula, percent full length product = (eff )(n-1)*100 where eff = coupling efficiency (for example, 99.4% = 0.994) and (n–1) is the number of coupling reactions needed to make an oligo of length n.
Figure 2. IDT tracks coupling efficiency at our manufacturing sites to ensure consistent quality. Many variables can lead to reduced oligo quality, including ambient temperature, humidity, and input reagents. Monitoring of coupling efficiency at IDT manufacturing sites ensures that we provide our customers with high-quality products. A quarterly average is shown (n = 16 per manufacturing site).
Resources
Frequently asked questions
What secondary structure considerations need to be included when designing primers for PCR?
The Tm for the strongest hairpin should be at least 50°C below the annealing temperature. These secondary structures and their corresponding stabilities can be calculated online with the IDT SciTools™ OligoAnalyzer Tool.
How many reactions will I be able to do with 25 nmol PCR primers?
For a typical 25mer oligonucleotide, 1 OD is equivalent to approximately 4 nmol, or 4000 pmol. Yield for a 20 base PCR primer on the 25 nmol scale is 12 nmol. Therefore, with even the minimum yield from a 25 nmol synthesis, you should be able to perform 1200 PCR reactions.
In summary: Minimum for a 20mer ≈ 12 nmol = 12,000 pmol = 1200 reactions.
Explore IDT's Custom Oligos
I am performing routine PCR, cloning, and amplicon sequencing. What scale should I use for oligo synthesis?
A 25 nmol scale, standard desalt oligonucleotide provides enough material for several hundred PCRs, so this scale should be sufficient.