Custom DNA & RNA Articles
An ASO modification that enhances nuclease resistance, lowers toxicity, and increases binding affinity
2′-MOE bases are often included in antisense oligos (ASO), aptamers, and siRNAs. Use them in place of standard RNA bases to increase nuclease resistance, reduce nonspecific protein binding, and to increase binding affinity with targeted complementary RNA.
- An ASO modification that enhances nuclease resistance, lowers toxicity, and increases binding affinity
- Buffers: What are they and how do they work?
- Primer design and other tools you should know about
- Colony PCR: What is it and why is it important
- Genome Oligopaint: Painting a clearer picture with oPools™ Oligo Pools
- Applications and benefits of CRISPR functional genomics screens using arrayed guide RNA libraries
- SHERLOCK, PAC-MAN, and more: How CRISPR-Cas13 enzymes may be useful in the COVID-19 pandemic and beyond
- Units of measure for oligo quantification
- Prime editing with pegRNA: a novel and precise CRISPR genome editing system
- What is CRISPR screening?
- Improve your assays with customizable oligos and probes containing cost-effective locked nucleic acids
- Four tips for oligonucleotide quantification with Thermo Scientific™ NanoDrop™ instruments
- Storing oligos: 7 things you should know
- Tips for resuspending and diluting your oligonucleotides
- Use of Template Switching Oligos (TS Oligos, TSOs) for efficient cDNA library construction
- N6-methyladenosine (m6A)—Modulating RNA localization, structure, stability, splicing, and translation
- Insert an abasic site into your sequence
- Increase the melting temperature of short, AT-rich primers and probes
- Oligo synthesis: Why IDT is a leader in the oligo industry
- Inverted bases
- RxnReady™ Primer Pools—Premixed oligos for PCR and more
- My oligos have arrived: Now what?
- Oligo modifications that block nuclease degradation
- When deoxythymine (dT) is required for modification attachment
- Calculating melting temperature (Tm)
- Calculations: Converting from nanograms to copy number
- Which type of oligo purification should I choose?
- Scale and yield—Are they the same?
- Oligo quantification—Getting it right
- Alternative dyes for FISH, FRET, and qPCR
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RUO23-1916_001