ATTO-labeled oligonucleotides offer additional alternatives for applications including fluorescence in situ hybridization (FISH), fluorescence resonance energy transfer (FRET), and dual-labeled probes used in qPCR experiments.* ATTO dyes are a group of patented fluorescent markers that feature strong absorption, photostability, and fluorescence quantum yield.
The ATTO dyes are perfect substitutes for dyes such as ROX™, VIC®, LIZ®, and Alexa Fluor® dyes. For qPCR applications, dye calibration will be needed for each qPCR instrument [1].
Ordering ATTO dyes
Catalog ATTO dyes can be attached to 100 nmol, 250 nmol, and 1 μmol DNA and RNA oligonucleotides, or duplex products. To order, visit the DNA Oligos or RNA Oligos ordering pages, where you can choose an ATTO dye as either a 5′ or 3′ modification. All ATTO-labeled oligos from IDT will be HPLC-purified.
Catalog ATTO dye |
Alternative for: |
---|---|
5' or 3' ATTO 488 (NHS Ester) | Alexa Fluor 488 |
5' or 3' ATTO 532 (NHS Ester) | VIC |
5' or 3' ATTO 550 (NHS Ester) | NED™ and Alexa Fluor 555 |
5' or 3' ATTO 565 (NHS Ester) | PET™ |
5' or 3' ATTO Rho101 (NHS Ester) | ROX™ |
5' or 3' ATTO 590 (NHS Ester) | Alexa Fluor 594 |
5' or 3' ATTO 633 (NHS Ester) | LIZ |
5' or 3' ATTO 647N (NHS Ester) | Alexa Fluor 647 |
For questions about ATTO dye modifications or products not found on this list, including internal dye placement or dual-labeled probes, reach out at contact us.
*ATTO dyes have not been tested on Illumina® platforms for use with certain fluorescent applications, such as sequencing, genotyping, and fragment analysis. Researchers must perform independent testing for such use.