Your product is now available from Integrated DNA Technologies.
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Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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Why is the Tm provided on your oligonucleotide specification sheets different from the calculated Tm I obtained when I designed my oligos?
Oligonucleotides designed using our online SciTools™ Web Tools are ordered for a wide variety of applications, and not solely for PCR. The Tm shown on the spec sheets is calculated based on default conditions of 50 mM Na+ without taking into consideration Mg2+ and dNTP concentrations, both which influence Tm. To determine the correct Tm or your oligo under your specific reaction conditions, enter the sequence into the online OligoAnalyzer Tool, making sure to change the defaults to your reactions conditions.