Integrated DNA Technologies acquires Archer™ next generation sequencing research assays to advance actionable scientific discoveries.

Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Search all FAQs:

What conditions should I use to HPLC purify my oligos?

IDT uses our own proprietary HPLC method to purify oligos in-house. A published set of conditions for an RP-HPLC protocol is:

  • Hamilton PRP-1 Reverse Phase column 250 x 10 mm
  • Buffer A = 100% ACN (acetonitrile)
  • Buffer B = ACN/0.1 M TEAA pH 7.3
  • Run at 4 mL/min over 30 min from 95–60% A
  • Monitor at 260/297 nm

Categories:
Custom DNA & RNA Optional services

Tags:
oligonucleotide primers purification