What can I do if I do not get any colonies during my cloning reaction using gBlocks™ Gene Fragments?

The most common source of this issue is an incomplete resuspension of your gBlocks Gene Fragment. The pellets can stick to the side or cap of the tube, and unless you vortex vigorously and incubate the tube at 50°C for 20 minutes, you might not have the DNA concentration you expect. We recommend the following:

1. Vortex your tube, and incubate at 50°C for 20 minutes.
2. Vortex vigorously again and spin the tube down.
3. Recheck the concentration of your solution. (In general, it is always a good idea to recheck your concentration after you resuspend a DNA sample.)