Integrated DNA Technologies acquires Archer™ next generation sequencing research assays to advance actionable scientific discoveries.

Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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Is titration of adapters required for different input amounts with the xGen™ DNA Library Prep Kit EZ / MC?

xGen DNA Library Prep Kit EZ

For direct sequencing of libraries prepared using the xGen DNA Library Prep Kit EZ from DNA inputs <25 ng, adapter dilution is necessary to achieve low levels of adapter dimers.

DNA input (ng)Reagent W4
≥25No dilution
 10 10-fold (1:10)
 1 20-fold (1:20)

xGen DNA Library Prep Kit MC

For direct sequencing of libraries prepared using the xGen DNA Library Prep Kit MC from DNA inputs <50 ng, adapter dilution is necessary to achieve low levels of adapter dimers. Adapter titration recommendations are outlined in the table below. Optimization may be required for diluting adapters of intermediate input scale.
 
DNA input (ng)Adapter
≥50No dilution
 10 10-fold (1:10)
 1 20-fold (1:20)

Categories:
Next generation sequencing

Tags:
ngs adapter Next generation sequencing