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For the xGen™ HS EGFR Pathway Amplicon Panel, we recommend a minimum of 10 ng input DNA. It is best to quantify input DNA using a qPCR-based assay since it reflects the amplifiable content of the sample.
Note that the input amount affects Limit of Detection (LOD) – 0.5% from 10 ng, and 0.25% from 20 ng.