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It depends upon your method for delivery, reaction volume, and Cas9 concentration.
As an example:
| Tube size | Delivery method | Reaction volume | Cas9 concentration | Number of reactions |
| 100 µg | Lipofection | 150 µL | 10 nM | ~400 |
| 100 µg | Electroporation | 10 µL | 3 µM | ~20 |