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For customers using the xGen™ SARS-CoV-2 Amplicon Panel who are comfortable with command line tools, and who want a ready-to-use variant calling analysis workflow that they can run on a local Linux machine, IDT offers a full variant calling workflow with all the tools and reference files pre-installed and configured in a Docker image available for download. See the xGen SARS-CoV-2 Amplicon Panel dockerized data analysis guidelines Tech Note for this information.
For details about primer trimming, review the Primerclip-A Tool for Trimming Primer Sequences App Note.